Fast Identification of Cyathula officinalis and Its Adulterant and Doped Adulterant Based on Properties and Foam Test / 中国实验方剂学杂志

Objective: To rapidly identify Cyathula officinalis and its adulterant C. capitata and C. officinalis*C. capitata and doped adulterant. Method: Properties combined with foam test method were used for identifying C.officinalis, its adulterant and doped adulterant. In the aspect of properties,6 aspects including shape,size,texture,color,smell and taste,were observed, smelled and tasted. In the aspect of foam test,the volume of foam produced was used as the determination index to investigate the sample amount,water amount, shaking time,particle size,water temperature,repeatability,adulteration ratio and its stability. Result: In the aspect of properties,C. officinalis and its adulterant showed obvious difference in the shape,size,color,texture,smell and taste,especially the red color and bitter taste of its adulterant. In the aspect of foam test,the optimum parameters were as follows:sample particle (screened with 3 sieves) 0.3 g,a test tube with plug and scale,water 10 mL and airtight,forced shaking up and down for 1 min,settling for 5 min. Such method can be used to identify C. officinalis, its adulterant and doped adulterant. The volume of foams produced by C. officinalis and its adulterant and different ratio of doped adulterant showed no change within 5-30 min,slightly decreases after 9 h; the higher adulteration ratio; the larger volume of foam and better stability. The 8 batches of C. officinalis and 8 batch of adulterants proved that the volume of the foams produced was all less than 2 mL in the C. officinalis,more than 13 mL in the adulterant is,and more than 5 mL in 5% doped adulterant, showing statistical difference. From the properties combined with foam test,5 specific identification elements were obtained for identifying C. officinalis, its adulterant and doped adulterant. Conclusion: Through the 5 specific identification elements,the properties combined with foam test can be used to distinguish the C. officinalis from its adulterant C. officinalis and C. officinalis*C. capitata and doped adulterant,characterized by accuracy,simpleness,short time,low cost and feasibility. It can provide a new method and reference for identifying C.officinalis from its adulterant and doped adulterant.

Identification of unclassified influenza A virus using high-throughput sequencing technology / 中华实验和临床病毒学杂志

Objective@#To identify the avian influenza virus subtype from the avian and environmental samples using the Ion Torrent new-generation semiconductor sequencing technology and to establish a high-throughput sequencing method to identify unclassified influenza A virus.@*Methods@#Virus RNA was extracted from the nine avian swab and environmental samples and real-time RT-PCR was carried out to detect universal fluA, H5N1, H7N9 and H9N2. The whole genome of influenza A virus was amplified by PathAmpFluA kit. Sequencing library was prepared using Next Fast DNA Fragmentation & Library Prep Set for Ion Torrent kit and high-throughput sequencing was done by Ion Torrent Personal Genome Machine(PGM). Data from the PGM was processed and quality evaluated using Ion TorrentSuite v3.0 software. Sequence assembly and influenza database blast were carried out by FluAtyping v4.0 and PathogenAnalyzer bioinformatics software to identify the influenza A virus subtype of these nine samples.@*Results@#The results of real-time RT-PCR for universal fluA of these nine samples were positive but the results for H5N1, H7N9 and H9N2 were negative. Seven subtypes of influenza A virus were identified by high-throughput sequencing and bioinformatics analysis: six samples were H2N3, H5N6, H5N8, H7N1, H7N7, H11N3 subtype respectively and three samples were H6N6 subtype.@*Conclusions@#Avian influenza virus has many subtypes in the environment of Zhejiang province. Ion Torrent semiconductor sequencing technology is suitable for fast identification of unclassified influenza virus for avian influenza environment monitoring.

Fast identification of constituents of Lagotis brevituba by using UPLC-Q-TOF-MS/MS method / 中国中药杂志

The chemical constituents of Lagotis brevituba were rapidly determined and analyzed by using ultra performance liquid chromatography tandem quadrupole time of flight mass spectrometry (UPLC-Q-TOF-MS/MS) method, providing material basis for the clinical application of L. brevituba. The separation was performed on UPLC YMC-Triart C₁₈ (2.1 mm×100 mm, 1.9 μm) column, with acetonitrile-water containing 0.2% formic acid as mobile phase for gradient elution. The flow rate was 0.4 mL•min-1 gradient elution and column temperature was 40 ℃, the injection volume was 2 μL. ESI ion source was used to ensure the data collected in a negative ion mode. The chemical components of L. brevituba were identified through retention time, exact relative molecular mass, cleavage fragments of MS/MS and reported data. The results showed that a total of 22 compounds were identified, including 11 flavones, 6 phenylethanoid glycosides, 1 iridoid glucosides, and 4 organic acid. The UPLC-Q-TOF-MS/MS method could fast identify the chemical components of L. brevituba, providing valuable information about L. brevituba for its clinical application.